Sophie Missen, 26, an occupational therapist working on a mental health acute ward, appears in the film.
She said: “I feel overwhelmed by this public show of gratitude.
“The last 12 months have been challenging but knowing the vital role we play in keeping people safe in the fight against the virus has kept me and my colleagues going.
“I feel humbled to represent my fellow frontline workers in this film.”
Bernadette Lavery, director of pharmacy at Boots UK, said: “We are grateful to all of the frontline workers who have worked tirelessly throughout the pandemic.
“But today, on International Women’s Day, we want to shine a light on the women who have continued to serve their local communities during this time.
“Boots has been supporting and empowering women for over 170 years, and with over 36,000 female colleagues within our business, we are particularly keen to say thank you to those women on the frontline today.”
The nation is being encouraged to continue to share thank you messages on International Women’s Day across social media using the hashtag #ThankyouFrontlineHeroes.